Comparison of dot-ELISA and standard ELISA for detection of Neisseria meningitidis outer membrane complex–specific antibodies

Comparison of dot-ELISA and standard ELISA for detection of Neisseria meningitidis outer membrane complex-specific antibodies.

Dot-ELISA using the outer membrane complex antigens of Neisseria meningitidis as a target was standardized for rapid detection of meningococcal-specific antibodies in human serum. We investigated the level of meningococcal-specific IgG, IgA, and IgM in serum using dot-ELISA with outer membrane antigens prepared from Neisseria meningitidis serotype B:4.19:P1.15,3,7,9 (a strain isolated from a Br...

Comparison of Dot-ELISA and Sandwich ELISA Diagnostic Tests in Detection of Human Hydatidosis

Hydatidosis, caused by the larval stage of Echinococcus granulosus, is one of the most important zoonosis with worldwide distribution. As its diagnosis by clinical symptoms and scanning alone is difficult and confusing, we designed the present study to achieve a sensitive and simple diagnostic method for epidemiological studies. Sera (250 samples) were collected from 90 cases of hydatidosis pro...

Comparison of Dot-ELISA with microbial culture for detection of Brucella spp. in clinical specimens

Definitive diagnosis of brucellosis is made by isolation of the causative agents, which is a timeconsumingprocedure. To evaluate the efficacy of Dot-ELISA for detecting brucellae in clinical samples, 94different specimens taken from animal origin were cultured on brucella selective culture media and colonieswere identified biochemically. The specimens were also examined after centrifugation by ...

Standardization of ELISA for Detection of Avian Influenza Virus Antibodies

development and standardization of dot - elisa for detection of neospora caninum antibodies in cattle and comparison with standard indirect elisa and direct agglutination test (dat).

background: neospora caninum is a protozoan parasite from phylum apicomplexa and an important agent causing abortion in cattle which produce notable economic loss all around the world. methods: dot-elisa was set up performing checker board procedure and then 178 sera of cattle examined with commercial indirect elisa and direct agglutination test (dat) were also evaluated by dot-elisa afterwards...